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A new imaging and micromanipulation tool for studying asymmetric cell division
par - 23 octobre 2007
A new single molecule technique will be developed, which combines the advantages of single particle tracking and magnetic tweezers. This new technique will enable the investigation of asymmetric cell division and cell polarity in vivo. Novel magneto-fluorescent nanoparticles will be used that allow for manipulation with magnetic gradients and simultaneous visualization of conjugated target molecules in living cells. Thus, it will be possible to generate intracellular activity gradient and to monitor the cell response.
Asymmetric cell division (ACD) is essential to generate cellular diversity during the development of multicellular organisms. For the unequal segregation of cell fate determinants the cell first needs to be polarized in response to intrinsic or extrinsic signals before mitosis. Therefore an axis of polarity is created before division, the mitotic spindle is oriented along the axis of polarity and the cell fate determinants are localized to one side of the cell. Thus, those determinants are inherited only to one of the daughter cells and as a consequence two distinct daughter cells are formed. Stem-cell like Drosophila neuroblasts (NBs) are an excellent model system for studying the mechanisms regulating ACD. They divide along their apical-basal axis and one daughter cell remains a NB while the other one becomes a ganglion mother cell.
Single particle tracking and magnetic tweezers are well-established single molecule techniques, which are ideally suited for in vivo applications with high spatiotemporal resolution. The combination of these two methods will give the unique possibility to tailor the spatial distribution of specific molecules and to monitor the spatiotemporal dynamics of the cell response.
Contacts : Maxime Dahan, Barbara Mueller.
Collaborations : Yohanns Bellaïche (Institut Curie, Paris), Moungi Bawendi (MIT, Cambridge, USA), Jacob Piehler (Goehte-University, Frankfurt, Germany)
